Yale New Haven Hospital

Department of Laboratory Medicine
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ANA IFA screen w/rflx titer and pattern, IFA

Lab Section: 



Lab Location: 

Main Lab

Test ID: 




Indirect Fluorescent Antibody (IFA)




3 days for screening, 4 days for titers

Specimen Requirements: 

Collection: 5.0 ml Blood in a Gold Top Tube.
Pediatric volume: 1 ml.
Unacceptable conditions: Grossly hemolyzed, or severely lipemic specimens
Stability: After separation from cells: Ambient: 2 days; Refrigerated: 7 days; Frozen: 3 months



Reflex Criteria: Negative- No further testing is done. Positive - Semi-Quantitative IFA titer is performed at an additional charge. Titer and Pattern are reported.

Clinical Indications: Anti-nuclear antibodies (ANA) are seen in a variety of systemic rheumatic diseases and are determined by indirect fluorescence assay (IFA) using HEp-2 substrate with an IgG-specific conjugate. ANA titers less than or equal to 1:80 have variable relevance while titers greater than or equal to 1:160 are often clinically significant. These antibodies may precede clinical disease onset; however, healthy individuals and those with advanced age have been reported to be positive for ANA. Patterns reported are: homogeneous, speckled, centromere, nucleolar, and nuclear dots. If cytoplasmic fluorescence is observed, it is noted. IFA methodology is subjective and has occasionally been shown to lack sensitivity for anti-SSA/Ro antibodies.

Reference Interval: 


CPT Code(s): 


Last Changed: 

12/17/2016 18:25:45

Last Reviewed: 

8/24/2017 2:30:58 PM