Yale New Haven Hospital

Department of Laboratory Medicine
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ANA, body fluid

Lab Section: 



Lab Location: 

Main Lab

Test ID: 




Indirect Fluorescent Antibody (IFA)




3 days for screening, 4 days for titers

Specimen Requirements: 

Collection: 3.0 ml Fluid in a Sterile Container.
Pediatric volume: 0.5 ml.
Please indicate fluid type.
Unacceptable conditions: Grossly hemolyzed specimens, viscous specimens.
Stability: After separation from cells: Ambient: 2 days; Refrigerated: 7 days; Frozen: 3 months



Reflex Criteria: Negative- No further testing is done. Positive - Semi-Quantitative IFA titer is performed at an additional charge. Titer and Pattern are reported.

Clinical Indications: Anti-nuclear antibodies (ANA) are seen in a variety of systemic rheumatic diseases and are determined by indirect fluorescence assay (IFA) using HEp-2 substrate with an IgG-specific conjugate. ANA titers less than or equal to 1:80 have variable relevance while titers greater than or equal to 1:160 are often clinically significant. These antibodies may precede clinical disease onset; however, healthy individuals and those with advanced age have been reported to be positive for ANA. When observed, one of the four basic patterns is reported: homogeneous, speckled, centromere, or nucleolar. If cytoplasmic fluorescence is observed, it is noted. IFA methodology is subjective and has occasionally been shown to lack sensitivity for anti-SSA/Ro antibodies.

Reference Interval: 


Tests in this Panel: 

Test Name

Reference Interval

ANA Titer Fluid [LRR11895]

Age Limit


Normal Range

0 Days - 999 Years


<_x0031_:_x0031_0 _x0028_Negative_x0029__x003c_ _x002f_td>

ANA Pattern [LRR11885]

ANA Screen, Fluid [LRR11890]

Age Limit


Normal Range

0 Days - 999 Years


<_x0031_:_x0031_0 _x0028_Negative_x0029__x003c_ _x002f_td>

CPT Code(s): 


Last Changed: 

12/17/2016 18:32:34

Last Reviewed: 

8/24/2017 2:49:58 PM