Yale New Haven Hospital

Department of Laboratory Medicine
Test Directory

[ Test Name Index ]

[ Home & References ]

Immunofixation electrophoresis, timed urine

Lab Section: 



Lab Location: 

Main Lab

Test ID: 




Qualitative Immunofixation Electrophoresis




5-7 days

Specimen Requirements: 

Collection: 20.0 mL Urine in a 24 Hr Urine Container.
Pediatric volume: 6 mL.
Refrigerate during collection.
Please send entire contents of timed urine container.
If an aliquot from a timed urine container is sent, collection duration and volume must be recorded.
Stability: Ambient: 2 days; Refrigerated: 1 week; Frozen: 1 month



Clinical Indications: A characteristic monoclonal band (M-spike) is often found in the urine of patients with monoclonal gammopathies. The initial identification of an M-spike or an area of restricted migration is followed by immunofixation to identify the immunoglobulin heavy chain and/or light chain. Immunoglobulin free light chains as well as heavy chain fragments may be seen in the urine of patients with monoclonal gammopathies. The presence of a monoclonal light chain M-spike can be consistent with a diagnosis of multiple myeloma or macroglobulinemia.

CPT Code(s): 


Last Changed: 

10/11/2016 17:58:24

Last Reviewed: 

08/23/2017 12:08:49