Yale New Haven Hospital

Department of Laboratory Medicine
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Special stains

Lab Section: 

Special Hematology



Lab Location: 

Main Lab


Test ID: 




Cytochemical Stain SPST


Cytochemical Stain


Mon-Fri days


1 day

Specimen Requirements: 

Collection: 3.0 ml Blood in a Lavender Top Tube.
Pediatric volume: 1.0 ml.
Performed only if >20% blasts in peripheral blood.
Handling: Blood/Bone Marrow; Ambient: 24 hours.
Unacceptable conditions: Fixed smears
Stability: Sample must be received within 24 hours of collection; testing must be performed within 48 hours of collection.



Reflex Criteria: All new acute leukemias reflex to an MD interpretation. Special Stains are added at the discretion of the CP/AP attending.

Clinical Indications: For characterization of blast lineage in acute leukemia.

See: PAS; Sudan Black B; Peroxidase(Myeloperoxidase); Alpha-Napthyl-Butyrate(Non-specific esterase).

Tests in this Panel: 

Test Name

Reference Interval

PAS Stain [LRR15457]

Interpretation Only

Peroxidase Stain [LRR15538]

Interpretation Only

Stain Source [LRR16374]


Alpha Naphthyl Butyrate Stain [LRR11769]

Interpretation Only

Sudan Black Stain [LRR16425]

Interpretation Only

CPT Code(s): 


Last Changed: 

8/17/2017 10:33 AM

Last Reviewed: 

8/17/2017 10:33 AM