Yale New Haven Hospital

Department of Laboratory Medicine
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ANA by IFA w/rflx to dsDNA, RNP, SM, SSA, and SSB Abs

Lab Section: 



Lab Location: 

Main Lab

Test ID: 




Semi-Quantitative Indirect Fluorescent Antibody (IFA)/ Semi-Quantitative Enzyme-Linked Immunosorbent Assay




3 days for screening, 4 days for titers

Specimen Requirements: 

Collection: 5.0 mL Blood in a Gold Top Tube.
Pediatric volume: 2 mL.
Unacceptable conditions: Grossly hemolyzed, or severely lipemic specimens
Stability: After separation from cells: Ambient: 2 days; Refrigerated: 7 days; Frozen: 3 months



Reflex Criteria: An ANA screen by IFA is performed. Negative, no further testing is done. Positive, semi-quantitative IFA titer is performed at an additional charge. If the titer is 1:160 or greater, DSDNA Ab, IgG with Crithidia IFA Confirmation and ENA Group (Sm, Sm/RNP, SS-A, and SS-B) will be performed at an additional charge.

Clinical Indications: Anti-nuclear antibodies (ANA) are seen in a variety of systemic rheumatic diseases and are determined by indirect fluorescence assay (IFA) using HEp-2 substrate with an IgG-specific conjugate. ANA titers less than or equal to 1:80 have variable relevance while titers greater than or equal to 1:160 are often clinically significant. These antibodies may precede clinical disease onset; however, healthy individuals and those with advanced age have been reported to be positive for ANA. When observed, one of the four basic patterns is reported: homogeneous, speckled, centromere, or nucleolar. If cytoplasmic fluorescence is observed, it is noted. IFA methodology is subjective and has occasionally been shown to lack sensitivity for anti-SSA/Ro antibodies.

Reference Interval: 


CPT Code(s): 


Last Changed: 

12/17/2016 18:22:55

Last Reviewed: 

8/24/2017 2:46:44 PM