Yale New Haven Hospital

Department of Laboratory Medicine
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Complement total, CH50

Lab Section: 



Lab Location: 

Main Lab

Test ID: 




Semi-Quantitative Enzyme-Linked Immunosorbent Assay




7 days

Specimen Requirements: 

Collection: 4.0 mL Blood in a Red Top Tube.
Pediatric volume: 1 mL.
Allow specimen to clot for one hour at room temperature. DO NOT REFRIGERATE.
Transport: After separation from cells, store serum frozen at -70C.
Unacceptable conditions: Samples collected in Gel Barrier Tubes; Grossly hemolyzed, lipemic or icteric samples.
Stability: After separation from cells: Refrigerated: Unacceptable; Frozen 2 weeks at -70C (preferably)



Clinical Indications: Low levels may occur during infections, disease exacerbation in patients with systemic lupus erythematosus, and in patients with immune complex diseases such as glomerulonephritis. Undetectable levels suggest the possibility of a complement component deficiency. Individual complement component assays are useful to identify the specific deficiency.

Reference Interval: 

0-60 CAE = Low activity
61-74 CAE = Borderline activity 
>=75 CAE = Normal activity

CPT Code(s): 


Last Changed: 

10/11/2016 09:21:48

Last Reviewed: 

8/18/2017 2:23:14 PM