Yale New Haven Hospital

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Paraneoplastic autoantibody evaluation

Lab Section: 

Sendout Reference

 

Lab Location: 

Sendout

Reference Lab: 

Mayo

 

Test ID: 

LAB4070

Synonyms: Anti-Hu,APCA,AChR

 

Methodology: 

Indirect Immunofluorescence Assay (IFA); reflexes to other methodologies if test reflexes.

Schedule: 

See individual tests for schedule.

Reported: 

20 days (confirmatory testing)

Specimen Requirements: 

Collection: 4.0 mL Serum Red Top. Acceptable: Serum gel
Pediatric volume: 2 mL.
Transport: Refrigerated.
Unacceptable conditions: Grossly hemolyzed, lipemic or icteric specimens.
Stability: Ambient: 72 hours; Refrigerated (preferred): 28 days; Frozen: 28 days

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

General Request Form (T239) (http://www.mayomedicallaboratories.com/it-mmfiles/general-request-form.pdf))

Neurology Specialty Testing Client Test Request (T732) (http://www.mayomedicallaboratories.com/it-mmfiles/neurology-request-form.pdf)

 

Notes: 

Reflex Criteria: If IFA (ANN1S, ANN2S, ANN3S, PCABP, PCAB2, PCATR, AMPHS, CRMS, AGN1S) patterns are indeterminate, paraneoplastic autoantibody Western blot is performed at an additional charge. If client requests or if IFA patterns suggest CRMP-5-IgG, CRMP-5-IgG Western blot is performed at an additional charge. If IFA pattern suggest NMO/AQP4-IgG, NMO/AQP4-IgG CBA is performed at an additional charge. If IFA patterns suggest amphiphysin antibody, amphiphysin Western blot is performed at an additional charge. If IFA patterns suggest GAD65 antibody, GAD65 antibody radioimmunoassay is performed at an additional charge. If IFA pattern suggest NMDA-R, NMDA-R Ab CBA and/or NMDA-R Ab IF Titer Assay is performed at an additional charge. If IFA pattern suggest AMPA-R, AMPA-R Ab CBA and/or AMPA-R Ab IF Titer Assay is performed at an additional charge. If IFA pattern suggest GABA-B-R, GABA-B-R Ab CBA and/or GABA-B-R Ab IF Titer Assay is performed at an additional charge. If ACh receptor binding antibody is >0.02, ACh receptor modulating antibodies and CRMP-5-IgG Western blot are performed at an additional charge. CRMP-5-IgG Western blot is also performed by specific request for more sensitive detection of CRMP-5-IgG. Testing should be requested in cases of subacute basal ganglionic disorders (chorea, Parkinsonism), cranial neuropathies (especially loss of vision, taste, or smell) and myelopathies. 4. If IFA patterns suggest GAD65 antibody, GAD65 antibody radioimmunoassay is performed at an additional charge. If ACh receptor binding antibody is >0.02 or if striational antibodies are > or = 1:60, ACh receptor modulating antibodies and CRMP-5-IgG Western blot are performed at an additional charge.

Clinical Indications: Serological evaluation of patients who present with a subacute neurological disorder of undetermined etiology, especially those with known risk factors for cancer Directing a focused search for cancer Investigating neurological symptoms that appear in the course of, or after, cancer therapy, and are not explainable by metastasis Differentiating autoimmune neuropathies from neurotoxic effects of chemotherapy Monitoring the immune response of seropositive patients in the course of cancer therapy Detecting early evidence of cancer recurrence in previously seropositive patients

See patient report or consult reference laboratory website

http://www.mayomedicallaboratories.com/test-catalog/Overview/83380

CPT Code(s): 

83519X6 83520x2, 86256

Last Changed: 

8/28/2017 6:16:37

Last Reviewed: 

8/28/2017 6:16:45