Yale New Haven Hospital

Department of Laboratory Medicine
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Gastric parietal cell antibody w/rflx titer

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Test ID: 




Parietal Cell Antibody


Enzyme Linked Immunosorbent Assay (ELISA)


Tuesday, Thursday pm


7 days

Specimen Requirements: 

Collection: 3.0 Collect 1 mL serum from a red top (no additive vial). Serum separator tube, plastic or glass, is also accepted.

Pediatric volume: 1 mL.
Transport: Refrigerated.
Unacceptable conditions: Gross hemolysis,  Lipemia , Received room temperature
Refrigerated 14 days; Frozen: 30 days



Clinical Indications: Anti-gastric parietal cell antibodies (Anti-GPA) were previously tested for by indirect immunofluorescence assay (IFA), using mouse stomach as a substrate. Identification of the specific antibody target as H+/K ATPase protein (a gastric proton pump) has led to the development of an ELISA based assay. Antibodies to this protein are present in approximately 80% of patients with pernicious anemia and a small percentage of the general adult population. The latter percentage increases with age and may reflect the presence of atrophic gastritis. A negative test does not exclude a diagnosis of pernicious anemia. A test for intrinsic factor blocking antibody (IFab) may provide serological evidence in support of the diagnosis in some of these patients.

Gastric Parietal Cell Antibodies are found in 90% of patients with pernicious anemia. They are also found in autoimmune chronic atrophic gastritis preceding pernicious anemia. The antigenic target is the H+/K+ ATPase gastric proton pump responsible for parietal cell acid generation in the stomach.

Reference Interval: 

See patient report or consult reference laboratory website.


CPT Code(s): 


Last Changed: 

8/28/2017 6:52:24

Last Reviewed: 

8/28/2017 6:52:33