Yale New Haven Hospital

Department of Laboratory Medicine
Test Directory

[ Test Name Index ]

[ Home & References ]

Gastric parietal cell antibody w/rflx titer

Lab Section: 

Sendout Reference

 

Lab Location: 

Sendout

Reference Lab: 

Quest

 

Test ID: 

LAB4193

 

Synonyms: 

Parietal Cell Antibody

Methodology: 

Enzyme Linked Immunosorbent Assay (ELISA)

Schedule: 

Tuesday, Thursday pm

Reported: 

7 days

Specimen Requirements: 

Collection: 3.0 Collect 1 mL serum from a red top (no additive vial). Serum separator tube, plastic or glass, is also accepted.

Pediatric volume: 1 mL.
Transport: Refrigerated.
Unacceptable conditions: Gross hemolysis,  Lipemia , Received room temperature
Stability:
Refrigerated 14 days; Frozen: 30 days

 

Notes: 

Clinical Indications: Anti-gastric parietal cell antibodies (Anti-GPA) were previously tested for by indirect immunofluorescence assay (IFA), using mouse stomach as a substrate. Identification of the specific antibody target as H+/K ATPase protein (a gastric proton pump) has led to the development of an ELISA based assay. Antibodies to this protein are present in approximately 80% of patients with pernicious anemia and a small percentage of the general adult population. The latter percentage increases with age and may reflect the presence of atrophic gastritis. A negative test does not exclude a diagnosis of pernicious anemia. A test for intrinsic factor blocking antibody (IFab) may provide serological evidence in support of the diagnosis in some of these patients.

Gastric Parietal Cell Antibodies are found in 90% of patients with pernicious anemia. They are also found in autoimmune chronic atrophic gastritis preceding pernicious anemia. The antigenic target is the H+/K+ ATPase gastric proton pump responsible for parietal cell acid generation in the stomach.

Reference Interval: 

See patient report or consult reference laboratory website.

 http://www.questdiagnostics.com/testcenter/TestDetail.action?ntc=15114

CPT Code(s): 

83516

Last Changed: 

8/28/2017 6:52:24

Last Reviewed: 

8/28/2017 6:52:33