Yale New Haven Hospital

Department of Laboratory Medicine
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Reticulin antibodies

Lab Section: 

Sendout Reference


Lab Location: 


Reference Lab: 



Test ID: 




ARA (Antireticulin Antibodies)


Indirect Immunofluorescence




7 days

Specimen Requirements: 

Collection: 3.0 mL Blood in a Gold Top Tube.
Pediatric volume: 1 mL.
Transport: Refrigerated.
Unacceptable conditions: Grossly hemolyzed, lipemic specimens.
Stability: Refrigerated(preferred): 14 days; Frozen: 14 days



Clinical Indications: Reticulin antibodies are no longer considered useful in the diagnosis of celiac disease (CD).Patients with CD produce various autoantibodies, including endomysial (EMA), tissue transglutaminase (tTG), gliadin, and reticulin antibodies, as part of the immune response. IgA antibodies usually predominate although patients may also produce IgG autoantibodies. The levels of these antibodies decline following institution of a gluten-free diet. tTG is the primary autoantigen recognized by EMA antibodies in patients with CD and is currently considered the most useful first level screening test for CD. Reticulin antibodies are no longer considered useful in the diagnosis of CD, because they lack the sensitivity and specificity of the EMA and tTG tests. Serological screening offers a minimally invasive option for rapid identification of those likely to have CD and to select those who should be subjected to biopsy. Markedly positive (serologically) individuals are highly likely to have CD and should undergo biopsy to confirm the diagnosis.

Reference Interval: 

See patient report or consult reference laboratory website.



CPT Code(s): 

86255 (screen), 86256 (titer if appropriate)

Last Changed: 

8/24/2017 12:26:05 PM

Last Reviewed: 

8/24/2017 12:26:16 PM